Ecoculture is an emerging focal concept reflecting the inextricability of nature and culture. It is applicable to and employed in many disciplines, yet it is rarely defined, cited, or interrogated, causing potential inconsistencies in scholarly operationalization. In the present analysis, I use Steven H. Chaffee's method of explication to develop an analytical review of ecoculture. I explore the primitive terms—ecology and culture—before assessing the scholarly use of the derived, compound term. I trace ecoculture across multiple disciplines, synthesizing operationalizations into one transdisciplinary theoretical framework. I find that ecoculture connotes interconnectedness and place relations, and has been critically operationalized in ways that problematize dominant human-centered ideologies, making it a productive scholarly frame that emphasizes the relationships between humans, their cultures, and their ecologies.